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NNMT acts as a primary metabolic gatekeeper, predominantly active in fat tissue and liver cells, where it methylates nicotinamide (NAM) using S-adenosyl-L-methionine (SAM) as a methyl donor

Sato, H., Tamba, M., Kuriyama-Matsumura, K., Okuno, S

Both A375 and B16F10 cells were treated with VH032, Alk-TIn, VH032 + Alk-TIn, VH032-Azi1 + Alk-TIn + SA, VH032-Azi2 + Alk-TIn + SA, and VH032-Azi3 + Alk-TIn + SA at 0.1 M for 12 and 24 h

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Sulforaphane, an isothiocyanate compound, is the most potent naturally occurring inducer of the body's key detoxification enzymes

5h), and standard drug group ( P = 0.0001